Measurement

Part:BBa_K1783003:Design

Designed by: Iowis Zhu   Group: iGEM15_UMaryland   (2015-08-14)

Unstable RFP under Hok/Sok Regulation


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 587
    Illegal NheI site found at 610
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1199
    Illegal AgeI site found at 1311
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Construct was assembled using Gibson assembly of a gBlock of K1783001 (Hok/Sok) and K1783002 (Const. Unstable RFP) into a pSB1C3 backbone. PCR amplification for primer extension of Hok/Sok component was difficult due to high inherent secondary structure of the gene sequences. Therefore, the Gibson Assembly of the Hok/Sok and Const. Unstable RFP proved to be difficult.

Source

Hok/Sok from K1783001 and constitutive unstable RFP from K1783002. Hok/Sok is from the E.coli R1 plasmid. RFP is from coral. The promoters are from the E.coli genome. LVA-tag described by Andersten et al.

References

1998, www.ncbi.nlm.nih.gov/pmc/articles/PMC106306/